Bacterial replication but not cell-to-cell spread is required for release of LDH. LDH release over time is shown. (A) Pam3CSK4-stimulated wt BMM were infected at an MOI of 5, and LDH release was assayed at 3, 4.5, and 6 h postinfection. (B) A total of 0.1 mg/ml chloramphenicol (cm) was added at 1, 2, 3, 4, or 5 h postinfection, and LDH release was measured at 6 h postinfection. Error bars represent standard deviations of the means. Data are representative of more than 3 independent experiments with similar results.