. 2011 Sep;79(9):3751–3759. doi: 10.1128/IAI.05182-11

Table 1.

Properties of the murine anti-fHbp MAbs

MAb (references)^c	IgG subclass	Bactericidal activity	Inhibition of fH binding	fHbp epitope
				Amino acid(s)^a	Binding to fHbp amino acid sequence variant^b
				Amino acid(s)^a	ID 1	ID 9	ID 74
JAR 3 (8, 48)	IgG3	Human or Rabbit C	+	Gly121, Lys122	++	++	+
JAR 5 (8, 48)	IgG2b	Rabbit C only	+	Gly121, Lys122	++	+	++
MAb502 (17, 41)	IgG2a	Rabbit C only	−	Arg204	++	++	−

fHbp mutants with amino acid substitutions at these positions lost or gained MAb binding (5, 8). In addition, for MAb502, the amide signals of Gly148, Arg149, and Ala174 showed chemical shifts by nuclear magnetic resonance (NMR) upon binding with fHbp (41).

Relative binding compared to fHbp ID 1 measured by quantitative Western blotting with integrated intensities of the bands measured by an infrared scanner (38). ++, ≥100% compared to ID 1; +, 52 to 62% compared to ID 1; −, <3% compared to ID 1.

The GenBank accession numbers for V_L and V_H genes of MAb502 are EU835941 and EU835942, respectively; those for JAR 5 are JF715927 and JF715926; and those for JAR 3 are JF715929 and JF715928.