Fig. 2.

Cell-distending activity of recombinant C. jejuni CDT subunits in CHO-K1 cells. CHO-K1 cells were left untreated (A) or treated with C. jejuni CDT holotoxin (CdtA, CdtB, or CdtC; 200 nM each subunit) (B) for 48 h at 37°C. The cells then were examined under an inverted optical microscope to assess the effects of CDT intoxication. Scale bar, 100 μm. The cell cycle distribution was based on the DNA content, which was determined by flow cytometry. The percentages of cells in the G₀/G₁, S, and G₂/M phases of the cell cycle are indicated below the insets. The results represent one of three independent experiments.