Skip to main content
. 2011 Sep;79(9):3770–3777. doi: 10.1128/IAI.05094-11

Fig. 1.

Fig. 1.

Schistosomes do not alter CD23 surface levels. (A) Circulating CD23+ B cell percentages were compared between subjects with schistosomiasis (n = 55) and North Americans (NA; n = 12). CD23+ B cells levels are higher in North Americans (P < 0.0001). (B) Purified splenic B cells were cultured in the presence of the indicated stimuli for 48 h or left untreated (No Tx). rIL-4 (20 ng/ml) and anti-CD40 (1 μg/ml) increase CD23, whereas crude schistosome antigens (SEA, 5 μg/ml; soluble egg antigen and SWAP, 5 μg/ml; soluble adult worm preparation) have no effect. rIL-10 (10 ng/ml) reduces basal levels of CD23. (C) Isolated peripheral lymph node cells were cultured in the presence of the indicated stimuli. rIL-4 (20 ng/ml), rIL-13 (10 ng/ml), anti-BCRμ (2 μg/ml), and Pam3CSK4 (TLR2 ligand; 1 μg/ml) increase CD23 levels, whereas IL-7 (20 ng/ml) has a null effect. (D and E) Purified circulating B cells from subjects with schistosomiasis were cultured in the presence of IL-4 and schistosome antigens (SEA and SWAP). IL-4 increased both the percentage (D) and the mean fluorescence intensity (MFI) (E) of CD23, but there was no effect from schistosome antigens (n = 13 to 19; P < 0.0001).