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. 2011 Sep;79(9):3821–3832. doi: 10.1128/IAI.05204-11

Fig. 6.

Fig. 6.

Proliferation of PBL from M. avium subsp. paratuberculosis (MAP)-exposed and -infected sheep and cattle. (A) Titration of the concentration of the IDO-blocking agent 1-MT (0 to 2 mM) in CFSE proliferation assays of cattle peripheral blood cells. Cultures were stimulated with M. avium subsp. paratuberculosis Ag, PWM, or medium alone as a control. Values are means ± SD of percentages of proliferation, determined by decreased CFSE fluorescence compared to that in nonproliferating controls. (B and C) Representative results of PBL from experimentally exposed animals are shown, specifically, proliferation of PBL from sheep (B) and cattle (C). Cultures were stimulated with M. avium subsp. paratuberculosis Ag, PWM, or medium with or without 1-MT. Values are means ± SD of percentages of proliferation in a CFSE assay. A representative unexposed control animal (sheep or cow) was compared to individual infected animals. Results for medium controls with and without 1-MT were not significantly different for all animals; therefore, only the medium-1-MT results are shown.