Fig. 2.
Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of TNF-α in response to LPS. Munc13-4jinx/jinx (jinx), Rab27aash/ash (ashen), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood samples were collected at 4 h or 24 h after injection. The samples were spun down, and plasma was collected and analyzed for the presence of TNF-α as described in Materials and Methods. A total of 7 mice for each group were analyzed in three independent experiments. The statistical significance of the difference of the means was calculated using the nonparametric Mann-Whitney test.