Fig. 4.

Liver sequestration of neutrophils during LPS-induced systemic inflammation requires Rab27a but not Munc13-4. Mice were injected intraperitoneally with LPS or PBS, and neutrophil infiltration into the liver was determined by analysis of tissue-associated myeloperoxidase at 4 h (A) and 24 h (B) after injections. Results are presented as means ± SEM. A total of 5 to 7 wild-type and ashen mice and 4 jinx mice for each condition were analyzed in three independent experiments. The statistical significance of the difference of the means was calculated using the nonparametric Mann-Whitney test. (C) Immunofluorescence analysis of neutrophil infiltration in liver at 4 h postinjection. Representative liver images from LPS-treated mice are shown. (D) Immunofluorescence analysis of neutrophil infiltration at 24 h postinjection of LPS. Infiltrated neutrophils (Ly-6G) are indicated with arrows. (E) Representative liver images from PBS-treated mice. Scale bars, 20 μm (C), 25 μm (E).