Fig. 8.
Mice lacking Rab27a or Munc13-4 have impaired in vivo secretion of MPO in response to LPS. Rab27aash/ash (ashen) (A) or Munc13-4jinx/jinx (jinx) (B) and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood samples were collected at 4 h after injection. The samples were spun down, and plasma was collected and analyzed for the presence of MPO using a mouse-specific ELISA. The statistical significance of the difference of the means was calculated using the nonparametric Mann-Whitney test (n = 6 mice, three independent experiments).