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. 1999 Mar 15;13(6):698–708. doi: 10.1101/gad.13.6.698

Figure 2.

Figure 2

Figure 2

(A) The 3.5-kb silenced HMR domain can be expanded to 7 kb. sirΔ strains carrying insertions (at SGD coordinate 293032) of either (1) 1-kb of the TRP1 gene (ROY49 and ROY1075) or (2) 1 kb of the TRP1 gene plus 2.5 kb of the HMG2 coding sequence (ROY55 and ROY1080) were generated. Strains ROY803, ROY84, ROY1076, and ROY 1079 are Sir+ derivatives of strains ROY49, ROY55, ROY1075, and ROY1080, respectively. All cells were grown in liquid media and 3 μl of 10-fold serial dilutions were spotted on either YPD plates (+Trp) or on YMD plates lacking tryptophan (−Trp) to assay for TRP1 expression. Strain numbers are shown in parentheses. (B) Differential restriction endonuclease digestion analysis of the expanded silenced HMR domain. Nuclei isolated from wild-type and sirΔ strains were digested with various restriction endonucleases. The DNA following purification was digested with a second restriction endonuclease and analyzed by DNA blot hybridization. For each site tested the band corresponding to wild-type cells is present on the left and the sirΔ strain is on the right.