Table 1.
Yeast strains
| Strain
|
Genotype
|
Strain
|
Genotype
|
|---|---|---|---|
| JRY19 | MATahis4-519 ura3Δ52 leu2-3,112 trp1 can1 | ROY1041* | MATα ADE2 lys2Δ HMRΔI mig1Δ::LEU2 |
| JRY3009 ROY113 | MATα ade2-1 MATα ade2-1 HMRΔ (nt. 288980 to 295350) | ROY1042* | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– mig1Δ::LEU2 |
| ROY49 | MATα lys2Δ sir4Δ::LEU2 HMR::TRP1 | ROY1044* | MATα ADE2 LYS2 HMRΔI spt8-302::LEU2 l |
| ROY508 | MATα ade2 LYS URA3–HMR ppr1Δ::HIS3 | ROY1046* | MATα ade2-1 LYS HMRΔI rlf6Δ::LEU2 |
| ROY513 ROY55 | MATα ade2 lys2Δ URA3–HMR ppr1Δ::HIS3 MATα lys2Δ sir4Δ::LEU2 HMR::TRP1::HMG2 | ROY1049* ROY1051* | MATαade2-1 LYS HMRΔI tec1Δ::HIS3 MATα ade2-1 LYS HMRΔI–(right bound insert)– |
| ROY648 | MATα ade2 LYS HMR::URA3 ppr1Δ::HIS3 | tec1Δ::HIS3 | |
| ROY652 | MATα ade2 lys2Δ HMR::URA3 | ROY1052* | MATα ADE2 lys2Δ HMRΔI spt4Δ::HIS3 ppr1Δ::HIS3 |
| ROY656 | MATa ADE2 lys2Δ sir3Δ::TRP1 HMR::URA3ppr1Δ::HIS3 | ROY1054* | MATαade2-1 LYS HMRΔI top2-1 |
| ROY687 ROY783 | MATα ADE LYS ppr1Δ::HIS3 HMR–URA3 MATa ADE ppr1Δ::HIS3 URA3–TEL–VR | ROY1059* ROY1060* | MATαade2-1 LYS HMRΔI pds1::LEU2 MATα ade2-1 LYS HMRΔI smc3-1::LEU2 |
| ROY787 | MATa ade2 ppr1Δ::HIS3 URA3–HMR–right bound–TEL–VR | ROY1061* | MATα ade2-1 LYS HMRΔI–(right bound insert)– smc3-1::LEU2 |
| ROY 791 | MATa ADE ppr1Δ::HIS3 URA3–HMR–right | ROY1063* | MATα ADE2 lys2Δ HMRΔI scc1-73 |
| bound–TEL–VR | ROY1065 | MATαade2-1 LYS HMRΔI pho4Δ::TRP1 | |
| ROY803 ROY834 | MATα ade2 LYS HMR::TRP1 MATα ADE lys2Δ URA3–HMR ppr1Δ::HIS3 sir3Δ::TRP1 | ROY1067 | MATα ade2-1 LYS2 HMRΔI–(right bound insert) pho4Δ::TRP1 |
| ROY836 | MATaade2 lys2Δ URA3–HMR ppr1Δ::HIS3 sir3Δ::TRP1 | ROY1075 | MATa ade2-1 LYS2 HMR::TRP1 sir3Δ::HIS3 |
| ROY838 | MATα ADE URA3–TRP1–TEL–VR–ppr1Δ::HIS3 | ROY1076 | MATa ade2-1 lys2Δ HMR::TRP1 |
| ROY84 ROY844 | MATα lys2Δ ade2 HMR::TRP1::HMG2 MATα ADE URA3–TRP1–TEL–VR–ppr1Δ::HIS3 | ROY1079 ROY1080 | MATaADE2 LYS2 HMR::TRP1::HMG2 MATa ADE2 LYS2 HMR::TRP1::HMG2 sir3Δ::HIS3 |
| ROY850 | MATalys2Δ ADE HMR–(right bound delete)–URA3 | ROY1088 | MATα ADE2 lys2Δ HMRΔI(right bound insert)– |
| sir3Δ::TRP1ppr1Δ::HIS3 | hat1Δ::HIS3 | ||
| ROY852 | MATα lys2D ADE2 HMR–(right bound delete)–URA3 | ROY1089 | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– |
| ppr1Δ::HIS3 | cac1Δ::LEU2 | ||
| ROY853 ROY935 | MATα lys2Δ ADE HMR–URA3 sir3Δ::TRP1 ppr1Δ::HIS3 MATa/MATα ppr1Δ::HIS3/ppr1Δ::HIS3 ADE/ADE | ROY1090 | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– rap1-12::LEU2 |
| LYS/LYS HMR/HMR–(right bound delete)–URA3 | ROY1094* | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– | |
| ROY938 | MATa/MATα ppr1Δ::HIS3/ppr1Δ::HIS3 ADE/ADE | rlf6Δ::LEU2 | |
| LYS/LYS HMR–(right bound delete)–URA3/HMR–(right bound delete)–URA3 | ROY1096* ROY1099* | MATαADE2 lys2Δ HMRΔI–(right bound insert)–top2-1 MATα ADE2 lys2Δ HMRΔI–(right bound insert)– | |
| ROY941 | MATa/MATα ppr1Δ::HIS3/ppr1Δ::HIS3 ADE/ADE LYS/LYS HMR–URA3/HMR–URA3 | ROY1101* | spt3-203::TRP1 MATα ADE2 lys2Δ HMRΔI–(right bound insert)– |
| ROY951 | MATα ade2-1 LYS2 HMRΔI | spt8-302::LEU2 | |
| ROY961 ROY1025 | MATα ADE2 lys2Δ HMRΔI–(right bound insert) MATα ade2-1 LYS HMRΔI cac1Δ::LEU2 | ROY1102* | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– pds1::LEU2 |
| ROY1027 ROY1029 | MATα ade2-1 LYS HMRΔI rap1-12::LEU2 MATα ADE2 LYS2 HMRδI hat1Δ::HIS3 | ROY1104* | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– scc1-73::TRP1 |
| ROY1032* ROY1034 | MATα ade2-1 LYS HMRΔI smc1-2::LEU2 MATα ADE2 lys2Δ HMRΔI rap1-13::LEU2 | ROY1108* | MATα ADE2 lys2Δ HMRΔI–(right bound insert)– smc1-2::LEU2 |
| ROY1036 | MATα ADE2 lys2Δ HMRΔI–(right bound insert) –rap1-13::LEU2 | ROY1112* | MATαADE2 lys2Δ HMRΔI–(right bound insert)– spt4Δ::HIS3 |
| ROY1039* | MATα ADE2 lys2Δ HMRΔI spt3-203::TRP1 |
All strains used in this study are either isogenic to W-303 or have been backcrossed six times with W-303 (*) and are therefore leu2-3,112 his3-11,15 ura3-1 trp1-1.