Fig. 2.

Alignment of Srl homologs. Homologs were identified by PSI-BLAST searches of the NCBI database using the CD2298 amino acid sequence from C. difficile CD630 as the query sequence. An E value of <1e−5 was chosen as a cutoff value for significance. Srl cognate proteins are present in other opportunistic pathogenic bacteria, including Anaerococcus and Fusobacterium species. The KIDEEL sequence in Srl is noted by red letters. A synthetic peptide sequence used for antibody production is underlined. Alignment is presented using CHROMA to yellow columns with 80% conservation. Consensus abbreviations (shown below alignment): h, hydrophobic (ACFGHILMTVWY); p, polar (CDEHKNQRST); s, small (ACDGNPSTV); c, charged (DEHKR); n, negatively charged (DE); +, positively charged (HKR).