Fig. 7.

The ER stress response is differentially activated in HK-2 cells in response to Stx1 or Stx2 treatment. HK-2 cells were exposed to 75 pg/ml Stx1 or Stx2, and at the indicated time points, cells were lysed and whole-cell lysates (100 μg/well) were subjected to SDS-PAGE (4% to 20%) and probed using antibodies specific for BiP (78 kDa) (A) and CHOP (28 kDa) (B). The blots shown are characteristic of three independent experiments. The bar graphs depict fold changes derived from mean densitometric readings of Western blot band intensities from three independent experiments. The data are expressed as means plus SEM, and statistical significance was calculated using one-way ANOVA. The asterisks denote significant differences compared to control cells (*, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant).