Fig. 2.

Relative gene expression of L. interrogans clpB, dnaK, and hsp10. Exponential-phase cultures of L. interrogans were incubated at 30°C (left columns) and 42°C (right columns) for 2 h. Transcripts of the selected genes were measured by RT-qPCR, and the data were analyzed using the comparative critical threshold (ΔΔC_T) method. The error bars indicate the standard deviations for three replicates.