Fig. 5.
Memory phenotypes of AMA-1-specific mouse lymphocytes generated after prime/boost vaccination. The percentage of CD62L- or CD44-positive proliferating cells (as indicated) was determined by flow cytometric analyses of splenocytes of BALB/c mice immunized according to the most relevant prime/boost protocols defined in this study and tested 14 weeks after the final immunization. CFSE-labeled splenocytes were induced to proliferate ex vivo for 48 h by adding PvAMA-1 (three lower rows of panels) or PvMSP-119 (negative controls, upper row of panels) to the cell cultures. Results are representative of two different experiments involving a total of six animals per group, individually analyzed.