Fig. 7.

MPLA treatment augmented sepsis-induced myeloperoxidase (MPO) production in macrophages but not neutrophils or double-positive myeloid cells. Mice were treated with MPLA or vehicle, followed by an intraperitoneal challenge with P. aeruginosa. (A) Representative dot plots from intraperitoneal cells that were recovered and assayed for F4/80 and Ly6G expression. (B) Histogram demonstrates intracellular MPO staining from the three myeloid populations in panel A (P1, F4/80⁺ monocytes; P2, Ly6G⁺ polymorphonuclear leukocytes; P3, F4/80⁺ and Ly6G⁺ immature myeloid cells). Isotype control, shaded curve; vehicle-treated mice, solid line; MPLA-treated mice, dotted line. The data are representative of three independent trials.