Fig. 1.

Recombinant DbpA proteins bind to biotinylated GAGs. Microtiter wells were mock coated (with PBS) or coated with His-tagged DbpA_N40,DbpA_B31, DbpA_PBr, or DbpA_VS461 and probed with biotinylated heparin, dermatan sulfate, or chondroitin-6-sulfate (see Materials and Methods). After a washing step, bound GAGs were detected by ELISA, as indicated by the absorbance at 450 nm. Each bar represents the mean of four independent determinations ± the standard errors. Asterisks indicate that GAG bound to DbpA-coated wells significantly (P < 0.05 [Student t test]) better than to mock-coated wells.