Fig. 6.

The C-terminal region of DbpA_VS461 is required for binding to GAGs and mammalian cells. (A) The C-terminal amino acid sequences of DbpA proteins analyzed in the present study. (B) Strain B314 expressing wild- type (WT) or mutant DbpA_VS461 were subjected to proteinase K (PK+) or mock (PK−) digestion and immunoblotted for DbpA or flagellin, as described for Fig. 1. Radiolabeled B314 expressing the vector control or the indicated DbpA_VS461 derivative was added to wells with 293 epithelial cells or no cells (C), or dermatan sulfate, decorin, chondroitin-6-sulfate, or BSA (D). The percentage of bacteria bound was determined by scintillation counting. Each bar represents the mean (± the standard errors) of four independent determinations.