Table 1.

Summary of EHEC 86-24 transcripts with a 1.5-fold or greater increase in expression after bile salt treatment relative to that of the untreated control^a

Gene	Product and predicted function	Fold change	Pvalue
ais	Protein induced by aluminum; function unknown	6.85	<0.005
arnC	Undecaprenyl phosphate-l-Ara4FN transferase	6.39	<0.001
arnD	Undecaprenyl phosphate-alpha-l-Ara4FN deformylase	4.46	<0.001
hycF	Formate hydrogen lyase complex iron-sulfur protein	3.43	<0.005
hycB	Hydrogenase 3, Fe-S subunit	3.21	<0.005
hydN	Putative electron transport protein HydN/iron-sulfur protein required for Hyd-3 activity	2.90	0.022
arnTb	l-Ara4N transferase	2.65	0.005
arnB	UDP-4-amino-4-deoxy-l-arabinose synthase; UDP-4″-ketopentose aminotransferase; l-glutamate is the amine donor	2.63	0.028
hycA	Transcriptional repression of hycand hypoperons	2.59	0.011
arnF	Undecaprenyl phosphate-alpha-l-Ara4N exporter; flippase ArnEF subunit	2.45	0.0066
acrR	acrABoperon repressor	2.37	0.015
fdhF	Formate dehydrogenase	2.36	0.014
ugdb	UDP-glucose 6-dehydrogenase	2.16	<0.001
basSb	Sensory histidine kinase in two-component regulatory system with BasR	2.13	0.0057
eptA	Predicted metal-dependent hydrolase/lipid A phosphoethanolamine transferase, associated with polymyxin resistance	2.12	0.031
hycI	Protease involved in processing of the C-terminal end of the large subunit of hydrogenase 3	2.05	<0.001
hycG	Component of hydrogenase 3; formate hydrogen lyase complex	2.04	0.01
arnA	UDP-glucuronate dehydrogenase and UDP-Ara4N formyltransferase	2.01	0.031
basR	DNA-binding response regulator in two-component regulatory system with BasS	2.00	<0.005
hycD	Formate hydrogen lyase complex inner membrane protein	1.91	0.026
arnF	Undecaprenyl phosphate-alpha-l-Ara4N exporter; flippase ArnEF subunit	1.90	<0.005
acrAb	Membrane fusion protein/component of AcrAB-TolC multidrug efflux system/acridine efflux pump	1.82	<0.005
acrB	AcrB RND-type permease/component of AcrAB-TolC multidrug efflux system	1.75	<0.005
hycC	Formate hydrogen lyase complex inner membrane protein	1.72	0.044
yeeF	Putative amino acid/amine transport protein; required for swarming phenotype, function unknown	1.65	<0.005
gatY	d-Tagatose 1,6-bisphosphate aldolase 2, catalytic subunit	1.56	0.019
hycE	Hydrogenase 3, large subunit	1.56	0.016
prmC	N5-Glutamine methyltransferase, modifies release factors RF-1 and RF-2	1.55	0.017
yfbQ	Predicted aminotransferase	1.51	<0.01
yehDb	Predicted fimbrial adhesin-like protein; FimA homologue	1.50	<0.01

^aAs determined by SAM analysis. n= 4 independent cultures (4 treatment, 4 control), n= 4 chips, n= 2 replicate spots per chip. Pvalues were determined using a one-way Student ttest. Bolded genes names indicate genes in operons in which increased expression was verified by β-galactosidase reporter assay.

^bIncreased expression verified by semiquantitative reverse transcriptase PCR.