Fig. 4.

(A) M. fumariolicumstrain SolV proteins in the crude extract were analyzed by 13% SDS-PAGE after subsequent centrifuging steps. (B) The proteins were then transferred onto a nitrocellulose membrane and tested for the presence of RuBisCO, using a peptide antibody against the large subunit of this enzyme. Lane numbers in both figures are the same. Lane 1, crude extract; lane 2, 12,000 × gsupernatant; lane 3, 48,000 × gsupernatant; lane 4, 150,000 × gsupernatant; lane C, control (crude extract without peptide antibody). The upper and lower bands indicated with arrows correspond to the large and small subunits of RuBisCO, respectively, identified by MALDI-TOF MS analysis.