Fig. 5.

(A) M. fumariolicumstrain SolV proteins in the crude extract were analyzed by 5% native PAGE after subsequent centrifuging steps. (B) The proteins were then transferred onto a nitrocellulose membrane and tested for the presence of RuBisCO, using the peptide antibody against the large subunit of this enzyme. Lane numbers in both figures are identical. Lane 1, crude extract; lane 2, 12,000 × gsupernatant; lane 3, 48,000 × gsupernatant; lane 4, 150,000 × gsupernatant. (C) The native band corresponding to the RuBisCO complex (indicated with an arrow in panel B) was analyzed on a 13% SDS-PAGE gel (lane 1).