Table 1.

Oligonucleotides used in this study

Oligonucleotide	Oligonucleotide sequence (5′–3′)	Applicationa
T653	TCTGCGAGTGTCTCGAAAAG	nrdAB5′ RACE primer
T602	CGCGAATTCCTCTTCTAGATGGGIIGGGIIGGGIIG	5′ RACE adapter primer
T694	TTCTAAAGCCTGAAAAATACGG	nrdABnested 5′ RACE primer
T1434	ATGCTGTGCCCGTTCTGCAA	CT406 5′ cloning primer
T1435	GGTCTGTTTTTCACCGTCCGG	CT406 3′ cloning primer
T1441	ATGCATTGCCCATTCTGTTTCGC	nrdR5′ cloning primer
T1442	GTCCTCCAGGCGCGCGAT	nrdR3′ cloning primer
T1137	AATTCACGTTGCTAGCTTCTATATATGGTATACAAGAGCCGAGACGTTCACAATATCTTGGGTTTTTAGGGGC	nrdABtandem NrdR box probe fwd
T1138	GATCCGCCCCTAAAAACCCAAGATATTGTGAACGTCTCGGCTCTTGTATACCATATATAGAAGCTAGCAACGTG	nrdABtandem NrdR box probe rev comp
T1326	AATTCCTAGATTTTCCTTTTGGTTGAGGATATAAATTCATTCT	ytgABCRDprobe fwd
T1327	GATCCAGAATGAATTTATATCCTCAACCAAAAGGAAAATCTAG	ytgABCRDprobe rev comp
T1350b	GCGCCTTTGTTGTATCGTCAGTTCAGGGTAAAATAGATTTCCGTTAACGTGTAGGCTGGAGCTGCTTC	nrdR5′ deletion primer
T1351	TCCTCGTTGCGCCAGCTTTAGCGCCCGCGCCATGTAATACTCGTCCTGCATATGAATATCCTCCTTAGTTCC	nrdR3′ deletion primer
T1490	CAGCAGAGGCGGATAAAAGT	luxA5′ cloning primer
T1513	GATCAGAAGAACGCTTTGAATTCATAAAATTG	luxA3′ cloning primer
T1491	TCAGAGGTTTTCACCGTCATC	luxB5′ cloning primer
T1512	CAATTTTATGAATTCAAAGCGTTCTTCTGATC	luxB3′ cloning primer
T1521	ATCGGTACCGGTTTCTTTTTGCCTGGTGA	nrdHIEFreporter 5′ cloning primer
T1522	CGTCTCGAGTTGATACAGAGATACTAGAT	nrdHIEFreporter 3′ cloning primer
T1523	CGAGGTACCGCTTAAAAGTTTGCTCTCGA	nrdABreporter 5′ cloning primer
T1524	TCGCTCGAGAGCCCCTAAAAACCCAAGAT	nrdABreporter 3′ cloning primer
T1599	TTCCTTTTGGTTGAGGATATAAATTCATTCTGTTAAAAGTATCTTTACAAT	ytgABCRDreporter insert fwd
T1600	ATTGTAAAGATACTTTTAACAGAATGAATTTATATCCTCAACCAAAAGGAA	ytgABCRDreporter insert rev comp
T1527	GGTGAATTCCTAGCACCTTCTATATATGGTATCTAAAATTCTTGACCATCACAATATCTTGGGTTATAATGACACCAACTT	dnaKpromoter + nrdABNrdR boxes fwd
T1528	AAGTTGGTGTCATTATAACCCAAGATATTGTGATGGTCAAGAATTTTAGATACCATATATAGAAGGTGCTAGGAATTCACC	dnaKpromoter + nrdABNrdR boxes rev comp
T1575	GAGGAATTCCTAGCACTTTGCTATATATTGTGTTAAAATTCTTGACCCAACTACATCTAGTACTATAATGACACCAACTT	dnaKpromoter + nrdHIEFNrdR boxes fwd
T1576	AAGTTGGTGTCATTATAGTACTAGATGTAGTTGTGGTCAAGAATTTTAACACAATATATAGCAAAGTGCTAGGAATTCCTC	dnaKpromoter + nrdHIEFNrdR boxes rev comp
T1689	AATTCACGTTGCTAGCATGTTGTTAAGCATTACAAGAGCCGAGACGTTCACAATATCTTGGGTTTTTAGGGGC	nrdABupstream NrdR box probe fwd
T1690	GATCCGCCCCTAAAAACCCAAGATATTGTGAACGTCTCGGCTCTTGTAATGCTTAACAACATGCTAGCAACGT	nrdABupstream NrdR box probe rev comp
T1693	AATTCACGTTGCTAGCTTCTATATATGGTATACAAGAGCCGAGACGTACTCTTAATGTACCGTTTTTAGGGGC	nrdABdownstream NrdR box probe fwd
T1694	GATCCGCCCCTAAAAACGGTACATTAAGAGTACGTCTCGGCTCTTGTATACCATATATAGAAGCTAGCAACGT	nrdABdownstream NrdR box probe rev comp

^afwd, forward; rev, reverse; comp, complement.