Global response of the A. vinelandiitranscriptome during diazotrophic growth. (A) Venn diagram comparing the total number of mRNA species undergoing at least a 2.0-fold change in transcript level under Mo-dependent diazotrophy (Nif, blue circle), under V-dependent diazotrophy (Vnf, green circle), and under heterometal-independent diazotrophy (Anf, rust circle) compared to a fixed-N replete (control) culture. Values in overlapping circles indicate the numbers of mRNA species that undergo a change in transcript level under both or all three conditions. nis the total number of mRNA species undergoing a change in transcript level in each culture. (B) Bar graph of the total number of mRNA species that undergo a larger-than-2-fold increase or decrease in transcript abundance as a result of metal availability and nitrogen limitation. Nif, Mo-dependent diazotrophy; Vnf, V-dependent diazotrophy; Anf, heterometal-independent diazotrophy; Control, N-replete culture.