Table 2.
PCR primers used in this study
| Primer | Sequence (5′ to 3′) | Restriction sitea |
|---|---|---|
| PH16-F | GGTACCATGATAATGGCTAAAGAATTACGTTTTGGT | KpnI |
| PH16-R | TCTAGACATCATTCCGCCCATGCCACCCAT | XbaI |
| htpB-Forward | GCCATTGCTCAAGTTGGAACTAT | None |
| htpB-Reverse | GCGTTGAAACCGTAGTTGTCTTT | None |
| CyaA-F | CCGGGGTACCATGCAGCAATCGCATCAGGCT | KpnI |
| CyaA-R | GCCTCTAGACGATCCCACCCCATCAAGGCT | XbaI |
| PBShtpB-F | TCTAGAATGATAATGGCTAAAGAATTACGTTTTGGT | XbaI |
| PBShtpB-R | GCATGCTTATTACATCATTCCGCCCATGCCACCCAT | SphI |
| GAL4htpB-F | GGAATTCCGTTTTGGTGATGAC | EcoRI |
| GAL4htpB-R | GCGTCGACTATTGGATAACCGGGAG | SalI |
| groELpEMBL-F | CGCGAGCTCATGGCAGCTAAAGACGT | SacI |
| pTrc99a-R | TCAGAAAGCTTCTGCGTTC | HindIII |
| mtHSP60-F | CGCGAGCTCATGTTGAGATCATCCGT | SacI |
| mtHSP60-R | CGGGATCCATCATACCTGGCATTCCT | BamHI |
| mthsp60Δ1-72-F | CGCGAGCTCATGAAAGAATTGAAATTCGGT | SacI |
| SPE2-F | CCCGGATCCATGACTGTCACCATAAAAGAAT | BamHI |
| SPE2-R | AATTCTGCAGTATTTTCTTCTGCAATTTC | PstII |
a
Restriction sites are shown as underlined boldface letters.