Fig. 1.

Growth properties of the wild-type PAO1 and of mutant strains in minimal medium supplemented with C₄-dicarboxylates. Growth curves of the wild-type PAO1 (⧫), the ΔdctAmutant PAO6592 (▵), the ΔdctPQMmutant PAO6708 (○), and the ΔdctAΔdctPQMmutant PAO6709 (▪) in BSM minimal medium containing succinate (A), fumarate (B), malate (C), or oxaloacetate (D) as the unique carbon source (40 mM) are shown. Each value is the average of three different cultures ± the standard deviation. In some instances, the standard deviation bars are smaller than the symbols used.