Table 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequencea |
|---|---|
| D2delchkF | GCATGACAACGCACGATTC |
| D2delchkR | CGAGATGCTGCGCCAATACG |
| Q266R_F | CGTCGGCGACGCCAACCGGACCATCTACTCGTTTACCG |
| Q266R_R | CGGTAAACGAGTAGATGGTCCGGTTGGCGTCGCCGACG |
| E508A_F | CACGCCGCCAAGGGACTGGCATGGGACGCGGTGTTCCTGG |
| E508A_R | CCAGGAACACCGCGTCCCATGCCAGTCCCTTGGCGGCGTG |
| D2southF | TGGACCGGTGTGCGTGCTGG |
| D2southR | ACTTGCTGTCCAGCAGCTGC |
| UvrD2int3 | TGCTTGCCGAGCTACGCCG |
| pKP186R | TCGCCACCTCTGACTTGAGC |
| pKP203R | CTACGTCGACATCGATAAGC |
| 7F | GGAATTCCATATGGAACTGGCATGACAACGCACG |
| 7R | GGAAGATCTCACGGCGAACTTGCTGTCCAG |
| 8F | GGAAGATCTGACGTGTGCAGCCGACG |
| 8R | TGCATGCATCCAGGTCGAGGTCTTCC |
| Hel_5_FLO | ROX-CATGGGCACCTAGGAGATCTCACGTACCCGTGGATCCTCTAGAGT |
| Hel_3_BHQ-2 | TGAGATCTCCTAGGTGCCCATG-BHQ-2 |
| Capture | CATGGGCACCTAGGAGATCTCA |
| 5′ Bio | Ct(B)GTCTATAGCGTGCAGTCACTTGAGCAT |
a
In oligonucleotides used for site-directed mutagenesis, base changes relative to the wild type are highlighted in gray. Restriction sites used for cloning are underlined.
t(B), biotinylated nucleotide.