The yeast ISWI complexes have distinct nucleosome
spacing activities. (A) Components required for the assembly
of regularly spaced nucleosome arrays using the yeast ISWI complexes.
Presence (+) and absence (−), respectively, of the components are
indicated. Partial and extended MNase digestions were taken for each
sample. (B) Titration of the yeast ISWI complexes in
nucleosome spacing assay. Threefold serial dilution of the yeast ISWI
complexes was used. All reactions contained NAP1, core histones, λ
DNA, and ATP at standard concentrations. Partial and extended MNase
digestions were performed for each sample.