Figure 6.

The yeast ISWI genes show genetic interactions. (A) The synthetic stress-sensitive phenotypes of mutants. Wild-type and mutant cells were cultured to saturation, and tenfold dilutions were made for spotting. The medium and the temperatures used are indicated. Formamide plate, containing 3% formamide in YPD, was incubated at 30°C. Relevant genotypes of the mutants are indicated in on left. (B) Rescue of a synthetic temperature-sensitive phenotype of the isw1 isw2 chd1 mutant. The triple mutant was transformed with the plasmids indicated at left, streaked on synthetic medium lacking uracil, and incubated at the temperatures indicated.