Fig. 3.
Recruitment of NER proteins to sites of local UV damage. Triple-color immunofluorescence analysis of NER factor recruitment to LUD spots in TTD-A and TTD-A corrected (TTDA-HA) cells. Three days prior to analysis, cells were seeded in a 1:1 ratio on coverslips. The cells were irradiated at 60 J/m2 through a filter containing 5-μm pores. The cells were fixed at different time points after UV irradiation (15 min and 1.5 and 4 h), and immunofluorescent staining was performed using antibodies against CPDs (blue) (marker for LUD), HA (green) (to discriminate between TTD-A and corrected TTD-A cells), and XPC (a), XPB (b), or XPA (c) (red).