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. 2011 Sep;31(17):3616–3629. doi: 10.1128/MCB.05164-11

Fig. 5.

Fig. 5.

PERK promotes survival in the luminal compartment via autophagy. (A) Representative confocal images of day 8 Fv2E-ΔNPERK and GFP-LC3 MCF10A acini treated from day 6 to 8 (+) or or not treated (−) with 100 pM AP, showing intraluminal LC3-puncta staining within cells (green). Middle panel, percentage of intraluminal LC3-puncta staining cells (n = 50); right panel, graph showing ATG gene mRNA transcript levels (middle panel) normalized to GAPDH. (B and C) Confocal equatorial images from Fv2E-ΔNPERK acini treated from day 6 to day 12 (+) or or not treated (−) with 100 pM AP and/or 20 μM chloroquine (CQ) (B) or shRNA ATG7 or control empty vector (EV) (C). The lower graphs show the distribution and mean number of intraluminal cells for each equatorial section of a single acinus (n = 20). Scale bar = 10 μm. Right blots show controls for CQ inhibition of autophagy (B) and ATG7 knockdown (C) in the 3D cultures. In all of the images, blue indicates DAPI staining of nuclear DNA, and unless otherwise indicated, scale bars indicate 25 μm. P values were determined by the t test.