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. 2011 Sep;31(17):3531–3545. doi: 10.1128/MCB.05124-11

Fig. 6.

Fig. 6.

AMPK activation in hypoxia: role of CaMKKβ. (A) LKB1−/− MEFs were transfected with negative-control siRNA (white bars) or siRNA for CaMKKβ (black bars) and exposed to hypoxia (1.5% O2) or normoxia (21% O2) for 2 h. Levels of AMPK phosphorylated at Thr-172 (p-AMPKα) and ACC phosphorylated at Ser-79 (p-ACC) as well as total AMPKα and ACC were measured by Western blotting. Graphs depict the fold change in the p-AMPK/AMPK or p-ACC/ACC ratio. Representative Western blots are shown. Values are expressed as means ± SEM (n = 5). *, P < 0.05 compared to normoxia control; †, P < 0.05 compared to hypoxia control. (B) The same protocol as for panel A was used to study the role of CaMKKβ in AMPK activation during hypoxia. Graphs depict the fold change in the p-AMPK/AMPK or p-ACC/ACC ratio. Representative Western blots are shown. Values are expressed as means ± SEM (n = 5). *, P < 0.05 compared to normoxia control; †, P < 0.05 compared to hypoxia control.