Figure 5.

Ternary complex formation with 5′ portion of rpoH mRNA as detected by toeprint analysis. (A) Urea–PAGE patterns of products of primer extension analysis. Toeprint assays with wild-type rpoH mRNA (nucleotides −60–+247) were carried out at 30°C or 42°C as described in Materials and Methods. The positions of full-length transcript and toeprint at +16 (as judged by sequence ladder run simultaneously) are indicated. Incubation times indicated (top) were different at the two temperatures. (B) Toeprint and full-length signals were quantified as described in Materials and Methods. (% toeprint) Calculated as the intensity of the toeprint signal divided by the sum of toeprint and full-length signals multiplied by 100.