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Mutations known to affect expression and thermoregulation in vivo also affect formation of the toeprint complex in vitro. Ternary complex formation with mutant mRNAs was performed at 30°C (A) or at 42°C (B). Toeprint and full-length signals were quantified. (% toeprint) Calculated as in Fig. 5B. Note that the values obtained with mutants other than 15A at 30°C are superimposed to that of the control in A.
