Table 2.

Roche-454-specific hits in plasma and CSF spiked with known viruses^a

Sample	No. of reads per sample	Avg length (nt)	No. of virus-specific hits per sample				Length (no. of virus-specific hits)b	Identity (%)
			RABV	PV	HHV-1	HHV-4
Plasma 10	27,516	247	0	0	0	NDc	ND	ND
Plasma 1000	23,009	264	5	0	4	ND	443	98
Plasma 100000	44,780	324	34	29	591	ND	403	99
CSF 10	33,453	352	0	0	0	ND	ND	ND
CSF 100	61,161	360	0	0	0	ND	ND	ND
CSF 1000	27,730	358	1	0	0	ND	438	99

^aFor each virus, the number of hits per sample is shown. The average length (nt) is given for all reads in a given sample and for the virus-specific hits. Percentages of identity were obtained after comparison to the reference sequences (for RABV and HHV-1 see text, for HHV-4, reference sequence GI:139424470).

^bWhen several viruses were detected in a sample, the length of the hits and the percent identity correspond to averages.

^cND, not determined.