Fig. 2.

Determination of the limit of detection for the Seeplex DV assay using quantified plasmid DNA. Raw (A) or analyzed (B) data for serial 10-fold dilutions of plasmid DNA for rotavirus, adenovirus, norovirus GI, and norovirus GII are depicted. Detection of amplified PCR product is evident at 45, 4.5, and 0.45 copies per reaction. Note that only a subset of the experiment representing each target is shown. In this experiment, triplicates of each dilution were tested in the same run. Note also that plasmid dilutions were retested on different days. Numbers in the table reflect band intensities.