Fig. 3.
Determination of the limit of detection for the Seeplex DV assay using quantified tissue culture fluid. Raw (A) or analyzed (B) data for serial 10-fold dilutions of tissue culture fluid for adenovirus, rotavirus, and norovirus GII are depicted. Detection of adenovirus amplicons is evident at a 10−6 dilution of the original concentration, whereas amplicons corresponding to rotavirus are detected at a 10−5 dilution and norovirus GII at a 10−4 dilution. Note that only a subset of the experiment representing each target is shown. In this experiment, triplicates of each dilution were tested in the same run. Note also that dilutions were retested on different days. Numbers in the table reflect band intensities.