Table 3.

Primers and probes used for target amplification

Target	Primer and probe sequencesb	Amp size (bp)c	Position	PCR method	Detection system	Reference
B. fragilis	f: GAG AGG AAG GTC CC	129	296–425	rRT-PCR	ABI7900	20
	r: CGC TAC TTG GCT GG
	p: FAM-CCA TTG ACC AAT ATT CCT CAC TGC TGC CT-BHQ
Norovirus GI	f: GCC ATG TTC CGI TGG ATG	76	5282–5358	rRT-PCR	ABI7900	11
	r: TCC TTA GAC GCC ATC ATC AT
	p: FAM- AGA TCG CGG TCT CCT GTC CA-BHQ
Norovirus GII	f: CAA GAG TCA ATG TTT AGG TGG ATG AG	77	5003–5080	rRT-PCR	ABI7900	11
	r: TCG ACG CCA TCT TCA TTC ACA
	p: CY5- TGG GAG GGC GAT CGC AAT CT-BHQ
Adenovirusa	f: CAG GAC GCC TCG GRG TAY CTS AG	103	—d	rRT-PCR	ABI7500	6
	r: GGA GCC ACV GTG GGR TT
	p: FAM-CCG GGT CTG GTG CAG TTT GCC CGC-BHQ
Rotavirusa	f:CCA TCT WCA CRT RAC CCT CTA TGA G	86	963–1049	rRT-PCR	ABI7500	35
	r:GGT CAC ATA ACG CCC CTA TAG C
	p: CY5-AGT TAA AAG CTA ACA CTG TCA AA-BHQ
Norovirus GI	Astra diagnostics (RealStar assay)	NA	NA	rRT-PCR	ABI7500	NA
Norovirus GII	Astra diagnostics (RealStar assay)	NA	NA	rRT-PCR	ABI7500	NA
Adenovirus	Seegene (Seeplex DV assay)	411	NA	2-Step RT-PCR	multiNA	NA
Astrovirus	Seegene (Seeplex DV assay)	650	NA	2-Step RT-PCR	multiNA	NA
Rotavirus	Seegene (Seeplex DV assay)	541	NA	2-Step RT PCR	multiNA	NA
Norovirus GI	Seegene (Seeplex DV assay)	304	NA	2-Step RT PCR	multiNA	NA
Norovirus GII	Seegene (Seeplex DV assay)	214	NA	2-Step RT PCR	multiNA	NA

^aThe primers used for both rotavirus and adenovirus are degenerate and allow for detection of multiple serotypes: R, A/G; Y, C/T; S, C/G; V, A or C or G; W, A/T.

^bf, forward; r, reverse; p, probe; FAM, 6-carboxyfluorescein.

^cAmp, amplicon.

^d—, base pair position for the adenovirus amplicon varies in different serotypes.