Table 6.
Calculated specificity and sensitivity of the Seeplex DV assay after discordant analysis
| Assay, virus, and result | No. of specimens with Seeplex DV result |
% sensitivitya | % specificityb | |
|---|---|---|---|---|
| True | False | |||
| Home brew multiplex rRT-PCR | ||||
| Adenovirus | ||||
| Positive | 28 | 1 | ||
| Negative | 1 | 168 | ||
| Total | 29 | 169 | 97 (28/29) | 99.4 (168/169) |
| Rotavirus | ||||
| Positive | 51 | 0 | ||
| Negative | 0 | 146 | ||
| Total | 51 | 146 | 100 (51/51) | 100 (146/146) |
| RealStar | ||||
| Norovirus GI | ||||
| Positive | 18 | 0 | ||
| Negative | 0 | 179 | ||
| Total | 18 | 179 | 100 (18/18) | 100 (179/179) |
| Norovirus GII | ||||
| Positive | 38 | 0 | ||
| Negative | 0 | 159 | ||
| Total | 38 | 159 | 100 (38/38) | 100 (159/159) |
a
Numbers in parentheses are no. positive by reference assay/total no. “true” by Seeplex DV.
b
Numbers in parentheses are no. negative by reference assay/total no. “false” by Seeplex DV.