Figure 2.

 Transcription of the LCR in K562 cells. (A) Diagram of the NRO probes used to detect transcription upstream of the ε-globin gene. The probes shown detect transcription in the direction of the ε-globin gene whereas the equivalent sense probes, denoted by the suffix S, detect antisense transcription in the opposite direction. (B) NRO analysis of LCR transcription in K562 cells in the absence and presence of α-amanitin. The corrected signals from the NRO performed in the absence of α-amanitin are shown graphically, with the exception of probe L4 that contains an Alu repeat. Analysis of transcription by use of sense probes is also shown. (C) NRO analysis of transcription in the 5′-flanking region of the ε-globin gene. The NRO data obtained from K562 cells by use of the shorter LCR probes, L13–L16, are shown with a graph of the corrected signals. The filter has been spliced to remove irrelevant probes.