Table 2.

Genotypic characteristics of 10 M. tuberculosis clinical MDR isolates and T_m deviations for different mutations obtained with the single real-time PCR assay

MDR isolate no.	Mutation site	Nucleotide substitution	Amino acid substitution	Tm (°C) deviation from wild typea
				rpoP1 (0.1)b	rpoP2 (0.2)	katP (0.1)	inhP (0.2)	ahpP (0.2)	embP (0.1)
125	rpoB513	CAA → AAA	Gln → Lys	4.4 ± 0.2c
	ahpC−12	C → T						4.4 ± 0.1
264	rpoB531	TCG → TTG	Ser → Leu		3.7 ± 0.2
	embB306	ATG → ATA	Met → Ile						10.1 ± 0.1
	ahpC−6	G → A						8.8 ± 0.1
278	rpoB511	CTG → CCG	Leu → Pro	9.1 ± 0.2
	rpoB513	CAA → CCA	Gln → Pro
	katG315	AGC → ACC	Ser → Thr			6.6 ± 0.3
	embB306	ATG → CTG	Met → Leu						7.4 ± 0.1
280	rpoB526	CAC → TAC	His → Tyr		2.6 ± 0.2
	ahpC−10	C → T						5.5 ± 0.0
286	rpoB531	TCG → TTG	Ser → Leu		3.7 ± 0.3
	ahpC−9	G → A						8.8 ± 0.2
	embB306	ATG → ATC	Met → Ile						11.1 ± 0.1
M31	rpoB526	CAC → AAC	His → Asn		2.6 ± 0.1
	katG315	AGC → ACC	Ser → Thr			6.4 ± 0.4
M32	embB306	ATG → GTG	Met → Val						4.0 ± 0.1
	rpoB516	GAC → GTC	Asp → Val	4.4 ± 0.1
	katG315	AGC → AAC	Ser → Asn			8.2 ± 0.4
M37	embB306	ATG → GTG	Met → Val						3.6 ± 0.1
	rpoB531	TCG → TTG	Ser → Leu		3.7 ± 0.1
	inhA−15	C → T					10.5 ± 0.1
M54	rpoB516	GAC → GTC	Asp → Val	4.8 ± 0.1
	rpoB533	CTG → CCG	Leu → Pro		5.2 ± 0.1
	ahpC−9	G → A						8.6 ± 0.1
M7	rpoB526	CAC → CTC	His → Leu		3.1 ± 0.1
	ahpC−6	G → A						8.8 ± 0.0

^aT_ms of the probes were obtained with a temperature increase rate of 1°C/s during the melting analysis.

^bStandard deviations of the T_ms for the six probes are given in parentheses.

^cValues are mean T_m deviations from three independent experiment repeats.

^dNegative numbers in designations indicate positions of mutations located before the start codon of the gene.