Table 1.
B. subtilis strains used in this studya
| Strain | Genotype and/or relevant featuresb | Source or reference |
|---|---|---|
| YB886 | B. subtilis 168 derivative freed of prophages PBSX and SPβ; wild-type strain; SPP1 indicator strain | 51 |
| YB886.Δ6 | YB886 derivative with deleted yueB (Δ6 deletion); contains an eryR marker inserted in the amyE locus; Eryr (0.5 μg ml−1) | This study |
| YB886.PspacYB | YB886 derivative expressing yueB under the control of the promoter Pspac (inducible with IPTG); Eryr (0.5 μg ml−1) | This study |
| YB886.Δ6.PxylYB | YB886.Δ6 derivative with PxylA-driven expression of yueB (inducible with xylose); Neor (7.5 μg ml−1) | This study |
| YB886.YBGFP | YB886 derivative driving native expression of yueB-gfp, yueBΩpCB-GFP; Spcr (100 μg ml−1) | This study |
| YB886.Δ6.PxylYBGFP | YB886.Δ6 derivative with PxylA-driven expression of yueB-gfp (inducible with xylose); Neor (7.5 μg ml−1) Spcr (100 μg ml−1) | This study |
| MMB357 | Expression of lacI-cfp; thrC::(Ppen-lacIΔ11-cfp (W7) mls Eryr (0.5 μg ml−1) Linr (12.5 μg ml−1) | A. D. Grossman |
| GSY10000 | YB886 derivative; expression of lacI-cfp; thrC::(Ppen-lacIΔ11-cfp(W7) mls Eryr (0.5 μg ml−1) Linr (12.5 μg ml−1) | This study |
a
See also Fig. 1.
b
Eryr, erythromycin resistance; Spcr, spectinomycin resistance; Neor, neomycin resistance; Linr, lincomycin resistance.