Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Relevant characteristic | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | Plasmid free, Lac− | Stratagene |
| S. aureus | ||
| RN4220 | Restriction deficient, prophage cured | 21 |
| Newman | Clinical isolate, L18P substitution in SaeS | 10 |
| USA300-0114 | Clinical isolate | NARSAa |
| USA300-P23 | USA300-0114 without plasmids 2 and 3 | This study |
| NMΔsae | sae operon deletion mutant of Newman | 42 |
| NM-P1m | Newman with P1 mutation | This study |
| USA-P1m | USA300-P23 with P1 mutation | This study |
| Plasmids | ||
| pYJ-lacZ | pYJ335 carrying a promoterless lacZ | 42 |
| pYJ-P3L-lacZ | pYJ-lacZ carrying P3L fragment | This study |
| pYJ-P3s-lacZ | pYJ-lacZ carrying P3s fragment | This study |
| pYJ-P3t-lacZ | pYJ-lacZ carrying P3t fragment | This study |
| pYJ-P3s10 m-lacZ | pYJ-P3s-lacZ with −12 and −13 substitutions | This study |
| pYJ-P3s35 m-lacZ | pYJ-P3s-lacZ with −35 and −36 substitutions | This study |
| pYJ-P3sTm-lacZ | pYJ-P3s-lacZ with −12, −13, −35, and −36 substitutions | This study |
| pCL-P3sGm-lacZ | pCL-P3s-lacZ with −9 substitution | This study |
| pCL-RSP | pCL55 carrying saeRS from Newman | This study |
| pCL-PQRSP | pCL55 carrying sae operon from Newman | 41 |
| pCL-RSL | pCL55 carrying saeRS from USA300 | This study |
| pCL-PQRSL | pCL55 carrying sae operon from USA300 | This study |
| pCL-R | pCL55 carrying saeR | This study |
| pCL-lacZ | pCL55 carrying promoterless lacZ | 42 |
| pYJ-Pcoa-lacZ | pCL-lacZ containing coagulase promoter | This study |
| pYJ-Phla-lacZ | pCL-lacZ containing alpha-hemolysin promoter | This study |
| pKOR1 | Allelic replacement plasmid | 3 |
| pKOR1-P1 m | pKOR1 carrying mutated P1 promoter fragment | This study |
| pAT18 | Shuttle vector for Gram-negative and Gram-positive organisms | 43 |
| pAT-P1-saeP | pAT18 carrying saeP under P1 promoter | This study |
| pAT-P1-saeQ | pAT18 carrying saeQ under P1 promoter | This study |
| pAT-P1-saePQ | pAT18 carrying saePQ under P1 promoter | This study |
| pKT25-saeR | pKT25 carrying saeR | This study |
| pUT18C-saeS | pUT18C carrying saeS | This study |
a
NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.