Table 1.
Strain/plasmid | Relevant characteristic | Source or reference |
---|---|---|
Strains | ||
E. coli DH5α | Plasmid free, Lac− | Stratagene |
S. aureus | ||
RN4220 | Restriction deficient, prophage cured | 21 |
Newman | Clinical isolate, L18P substitution in SaeS | 10 |
USA300-0114 | Clinical isolate | NARSAa |
USA300-P23 | USA300-0114 without plasmids 2 and 3 | This study |
NMΔsae | sae operon deletion mutant of Newman | 42 |
NM-P1m | Newman with P1 mutation | This study |
USA-P1m | USA300-P23 with P1 mutation | This study |
Plasmids | ||
pYJ-lacZ | pYJ335 carrying a promoterless lacZ | 42 |
pYJ-P3L-lacZ | pYJ-lacZ carrying P3L fragment | This study |
pYJ-P3s-lacZ | pYJ-lacZ carrying P3s fragment | This study |
pYJ-P3t-lacZ | pYJ-lacZ carrying P3t fragment | This study |
pYJ-P3s10 m-lacZ | pYJ-P3s-lacZ with −12 and −13 substitutions | This study |
pYJ-P3s35 m-lacZ | pYJ-P3s-lacZ with −35 and −36 substitutions | This study |
pYJ-P3sTm-lacZ | pYJ-P3s-lacZ with −12, −13, −35, and −36 substitutions | This study |
pCL-P3sGm-lacZ | pCL-P3s-lacZ with −9 substitution | This study |
pCL-RSP | pCL55 carrying saeRS from Newman | This study |
pCL-PQRSP | pCL55 carrying sae operon from Newman | 41 |
pCL-RSL | pCL55 carrying saeRS from USA300 | This study |
pCL-PQRSL | pCL55 carrying sae operon from USA300 | This study |
pCL-R | pCL55 carrying saeR | This study |
pCL-lacZ | pCL55 carrying promoterless lacZ | 42 |
pYJ-Pcoa-lacZ | pCL-lacZ containing coagulase promoter | This study |
pYJ-Phla-lacZ | pCL-lacZ containing alpha-hemolysin promoter | This study |
pKOR1 | Allelic replacement plasmid | 3 |
pKOR1-P1 m | pKOR1 carrying mutated P1 promoter fragment | This study |
pAT18 | Shuttle vector for Gram-negative and Gram-positive organisms | 43 |
pAT-P1-saeP | pAT18 carrying saeP under P1 promoter | This study |
pAT-P1-saeQ | pAT18 carrying saeQ under P1 promoter | This study |
pAT-P1-saePQ | pAT18 carrying saePQ under P1 promoter | This study |
pKT25-saeR | pKT25 carrying saeR | This study |
pUT18C-saeS | pUT18C carrying saeS | This study |
NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.