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Determination of pKa values for the interchange thiol of 2-KPCC. The fold increase in A555 due to formation of charge transfer absorbance between FAD and the thiolate of C87 is plotted versus pH. The lines were derived from the four-parameter sigmoidal curve fit used to calculate pKa values. Symbols: •, C82A mutant of r-2-KPCC; ▵, native 2-KPCC alkylated on C82 with BES.
