Fig. 5.

Dependence of potassium efflux on GSH and KefF with benzoquinone as an electrophile. (A) The GSH-deficient strain Frag56 was grown in the presence (red) and absence (black) of GSH in the growth medium, and the potassium efflux triggered by benzoquinone was measured afterward. (B) The dependence of the potassium efflux on KefF was tested with the kefF mutant strain MJF654, which was used either untransformed or transformed with WT KefF-, G107S-, or H112W-bearing plasmids. Rate constants for the potassium efflux are given.