Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| Strains | ||
| Frag5 | F− ΔkdpABC5 thi rha lacZ | 9 |
| Frag56 | Frag5 gshA::Tn10 (kan) | 8 |
| MJF274 | F− ΔkdpABC5 thi rha lacZ trkD1 | 8 |
| MJF276 | MJF274 kefB157 kefC::Tn10 | 33 |
| MJF362 | Frag5 Δ[yabF-kefC]::kan | 29 |
| BW25113 ΔkefF | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) lambda−rph-1 Δ(rhaD-rhaB)568 hsdR514 kefF::kan | This study |
| MJF654 | BW25113 kefF::kan kefBG::apr | This study |
| Plasmids | ||
| pTrcYabFH6 | KefF from E. coli with C-terminal His6 tag | This study |
| pYabFH6G107S | E. coli KefF with G107S mutation preventing FMN binding | This study |
| pYabFH6H112W | E. coli KefF with H112W mutation blocking substrate binding | This study |
| pYabFH6F149W | E. coli KefF with F149W mutation blocking substrate binding | This study |
| pYabFH6C147S | Mutation evaluating the regulatory role of surface-exposed cysteine | This study |
| pYabFH6C151S | Mutation evaluating the regulatory role of surface-exposed cysteine | This study |
| pYabFH6C147S/C151S | Mutation evaluating the regulatory role of surface-exposed cysteine | This study |