Regulation of expression of lacZ reporter gene fusions by zinc in mutant strains. Cells of C. metallidurans AE104 (•) mutant strains were incubated in TMM containing various concentrations of Zn2+. These strains carried lacZ fusions of pitA (A), corA1 (B), corA2 (C), or corA3 (D). The mutant strains were a ΔzupT deletion strain (○) (A to D) and a ΔpitA (□) and a ΔpitA ΔzupT (▵) deletion strain (B, C, and D). At least three independent determinations were made. The error bars indicate standard deviations.