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Zinc and phosphate contents of C. metallidurans and E. coli cells. The contents of zinc and phosphate atoms per cell were determined by ICP-MS in C. metallidurans CH34 wild type (•), megaplasmid-free AE104 (▪), and the AE104 ΔzupT (○) and ΔzupT ΔpitA (□) mutant strains, as well as E. coli strain W3110 (♦). The cells were cultivated for 20 h in TMM with shaking at 30°C. Three independent determinations were made. The error bars indicate standard deviations.
