Table 3.

Uptake of ⁶⁵Zn²⁺ by various C. metallidurans mutant strains

Strain or mutant	Uptake of 65Zn2+a
	10 μM Mg2+		1 mM Mg2+
	Without SL6	With SL6	Without SL6	With SL6
AE104	1.15 ± 0.60	0.83 ± 0.07	0.19 ± 003	0.11 ± 0.01
ΔzupT	1.15 ± 0.18	0.91 ± 0.13	0.11 ± 0.04	0.33 ± 0.26
ΔpitA	0.86 ± 0.03	1.12 ± 0.02	0.42 ± 0.13	0.52 ± 0.25
corA1::kan	1.10 ± 0.28	1.11 ± 0.18	0.22 ± 0.07	0.20 ± 0.06
ΔcorA2	1.13 ± 0.03	1.00 ± 0.02	0.20 ± 0.06	0.22 ± 0.03
ΔcorA3	1.45 ± 0.24	0.90 ± 0.10	0.18 ± 0.04	0.24 ± 0.07
ΔzupT ΔpitA	1.06 ± 0.34	0.95 ± 0.09	0.44 ± 0.34	0.37 ± 0.14

^aAs for Fig. 6, C. metallidurans strain AE104 and its mutant derivatives were cultivated in TMM with decreased magnesium content (10 μM) (Fig. 6A and B) or 1 mM Mg²⁺ (Fig. 6C and D), either without (Fig. 6A and C) or with (Fig. 6B and D) the trace element solution SL6 in the medium. The cells were harvested by centrifugation, suspended in Tris buffer, and used in uptake experiments with ⁶⁵Zn²⁺. The values at a t of 2 min are given in μmol ⁶⁵Zn²⁺/g dry mass. At least 3 reproductions.