Fig. 5.

Northern blot analysis of ectT transcription in response to salt or cold stress. Cultures of V. pantothenticus were grown in SMM to mid-exponential growth phase (OD₅₇₈ of about 1) at 37°C and then subjected to an osmotic up-shock with 0.7 M NaCl (A) and a sudden temperature downshift to 15°C (B). Cells were harvested at the indicated time points, and the ectT transcript was visualized through hybridization with a digoxigenin-labeled single-stranded antisense RNA probe.