Figure 9.

kek-1 is expressed in follicle cells sensitive to loss of dlg activity. Egg chambers harbor a single copy of a lacZ enhancer trap gene inserted in the 5′ noncoding region of kek-1 (Musacchio and Perrimon 1996). kek-1 expression is revealed by X-gal staining. (a) kek-1 is intensely expressed at the anterior and posterior poles of egg chambers throughout the time that follicle cells are proliferating, from s1 to s6, and continuing to s7 (arrows). At s8, kek-1 is expressed in the presumptive BCs at the anterior of the egg chamber (arrowhead). At s9, kek-1 is expressed in migrating BCs, and in follicular epithelial cells covering the oocyte, but not in nurse cell-associated epithelial cells. (b) Higher magnification of s4–s7 egg chambers shown in (a). In addition to polar staining, kek-1 is expressed at lower levels in lateral follicle cells (arrowheads). (c) Multiple layers of follicle cells at the anterior and posterior poles of dlg^sw egg chambers express kek-1. (d) Invasive follicle cells in dlg^hf321/dlg^lv55 egg chambers express kek-1.